Australia’s critically endangered alpine tree frogs use sex to fight killer fungus
Infected males produce higher-quality sperm, display brighter throat patches and sire nearly a third more offspring

Ima CaldwellFri 6 Jun 2025 in The Guardian
The number of critically endangered alpine tree frogs, found only in the Australian alps, has crashed by about 80% since the 1980s.
Populations have been hit by chytrid fungus, a disease that has devastated amphibian populations globally. But a new study has found a surprising silver lining that – for now – is helping the species hang on in the face of extinction.
Researchers at the University of Melbourne found that male alpine tree frogs with the deadly chytridiomycosis breed more prolifically than healthy frogs. The infected frogs produce higher-quality sperm, display brighter throat patches during mating displays and sire nearly a third more offspring.
Researchers believe it’s a trade-off: the frogs shift their energy from fighting infection to reproducing – a last-ditch evolutionary push to pass on their genes.
The phenomenon is rare in the animal kingdom but not unheard of. Similar behaviour has been observed in female Tasmanian devils with facial tumours, which tend to reproduce earlier and in greater numbers.
Still, the authors of the alpine frog study say the trend is not a long-term safeguard against extinction.
“This increased reproduction is able to offset the mortality, but it doesn’t help them build their populations back up,” said lead researcher Dr Laura Brannelly.
Once common throughout the alpine regions of Victoria and New South Wales, Litoria verreauxii alpina now survives in just eight fragmented strongholds across both states.
Chytrid fungus spreads through water. It infects the outer layer of the frog’s skin, disrupting their system for regulating respiration, water and electrolytes and ultimately killing them via cardiac arrest.
It is blamed for extinction of at least seven Australian frog species – including the gastric-brooding frog, famous for giving birth through its mouth and last seen in 1981. While disease remains the primary driver of the species’ decline, it is not the only threat they face.
Brannelly said habitat loss, drought and bushfires are compounding the risk. Global heating is drying out boggy alpine wetlands, which the frogs rely on for breeding grounds, she said, and four-wheel driving, dam construction, hunting and fishing have all damaged fragile habitats.
“There’s a lot of recreation up there,” she said. “If we can support those groups to also be creating spots that support alpina, plus the recreation, then we can hit all the marks.”
Her team’s research recommends creating artificial breeding ponds and corridors between the frog populations, to help boost the species’ chances of survival.
Tracking the frogs to gather the study’s data was not easy. Brannelly and her team spent nights driving through Mount Hotham in the Victorian Alps, periodically turning off the engine to listen for the gravelly whistle of the male’s mating call – a sound that used to echo through the region.
They followed the sound and waded through bogs and snowmelt streams to find the frogs – tiny, cold and remarkably hardy.
Growing up to just 3cm long, alpine tree frogs range in colour from bright green to mottled brown, often with spots or stripes.
They blend almost seamlessly into their surroundings. Their most extraordinary trait, however, might be their ability to survive after being frozen, something researchers still do not fully understand.
“Sometimes you find one and you think, ‘Oh my God, there’s a frozen dead frog’ – and then it will just come back to life,” Brannelly said.
But even with this resilience, the species is under significant pressure.
Nick Clemann, a senior herpetology biologist at Zoos Victoria, said the frogs’ survival is “on a knife’s edge”.
“The alpine tree frog used to be widespread and found in high density,” he said. “Before the disease hit, you would have heard lots of males calling in the wild.”
They are typically hardy, with tadpoles found swimming even in water pooled inside discarded tyres.
Clemann said the frog’s disappearance from alpine ecosystems, where few cold-blooded animals live, was “catastrophic.”
Frogs play a crucial ecological role, controlling insect populations, serving as prey for birds, fish and other wildlife and filtering water as tadpoles.
The alpine frog is listed as endangered in NSW and critically endangered in Victoria, giving impetus to conservation efforts. But Clemann said the real work is happening at ground level, driven by “dedicated individuals and organisations”.
“That’s where the difference is made.”
Clemann agreed with Brannelly that the most practical path forward is habitat modification – for conservationists to create artificial environments to support breeding habitat.
He believes protecting the alpine tree frog and its habitat requires practical collaboration between traditional custodians, regulators such as Parks Victoria and recreational visitors to the habitat.
References
- 1. Preece ND et al.. 2017 A guide for ecologists: detecting the role of disease in faunal declines and managing population recovery. Biol. Conserv. 214, 136–146. (doi:10.1016/j.biocon.2017.08.014) Crossref, Web of Science, Google Scholar
- 2. González-Barrio D, Pruvot M, Kock RA, Fernández Aguilar X. 2023Editorial. Anthropogenic wildlife movements and infectious diseases: health and conservation perspectives. Front. Vet. Sci. 10, 1132176s10393-014. (doi:10.3389/fvets.2023.1132176) Crossref, Web of Science, Google Scholar
- 3. Airey M, Short KR. 2024 High pathogenicity avian influenza in Australia and beyond: could avian influenza cause the next human pandemic? Microbiol. Aust. 45, 155–158. (doi:10.1071/ma24040) Crossref, Google Scholar
- 4. Langwig KE et al.. 2015 Host and pathogen ecology drive the seasonal dynamics of a fungal disease, white-nose syndrome. Proc. R. Soc. B 282, 20142335. (doi:10.1098/rspb.2014.2335) Link, Web of Science, Google Scholar
- 5. Scheele BC et al.. 2019 Amphibian fungal panzootic causes catastrophic and ongoing loss of biodiversity. Science 363, 1459–1463. (doi:10.1126/science.aav0379) Crossref, PubMed, Web of Science, Google Scholar
- 6. Berger L. 1998 Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America. Proc. Natl Acad. Sci. USA 95, 9031–9036. (doi:10.1073/pnas.95.15.9031) Crossref, PubMed, Web of Science, Google Scholar
- 7. Grogan LF, Robert J, Berger L, Skerratt LF, Scheele BC, Castley JG, Newell DA, McCallum HI. 2018Review of the amphibian immune response to chytridiomycosis, and future directions. Front. Immunol. 9, 2536. (doi:10.3389/fimmu.2018.02536) Crossref, PubMed, Web of Science, Google Scholar
- 8. Brannelly LA et al.. 2021Mechanisms underlying host persistence following amphibian disease emergence determine appropriate management strategies. Ecol. Lett. 24, 130–148. (doi:10.1111/ele.13621) Crossref, PubMed, Web of Science, Google Scholar
- 9. Brannelly LA, Webb R, Skerratt LF, Berger L. 2016 Amphibians with infectious disease increase their reproductive effort: evidence for the terminal investment hypothesis. Open Biol. 6, 150251. (doi:10.1098/rsob.150251) Link, Web of Science, Google Scholar
- 10. Brannelly LA, Webb RJ, Jiang Z, Berger L, Skerratt LF, Grogan LF. 2021 Declining amphibians might be evolving increased reproductive effort in the face of devastating disease. Evolution 75, 2555–2567. (doi:10.1111/evo.14327) Crossref, PubMed, Web of Science, Google Scholar
- 11. Chatfield MWH, Brannelly LA, Robak MJ, Freeborn L, Lailvaux SP, Richards-Zawacki CL. 2013 Fitness consequences of infection by Batrachochytrium dendrobatidis in northern leopard frogs (Lithobates pipiens). EcoHealth 10, 90–98. (doi:10.1007/s10393-013-0833-7) Crossref, PubMed, Web of Science, Google Scholar
- 12. Valenzuela-Sánchez A et al.. 2022Interpopulation differences in male reproductive effort drive the population dynamics of a host exposed to an emerging fungal pathogen. J. Anim. Ecol. 91, 308–319. (doi:10.1111/1365-2656.13603) Crossref, PubMed, Web of Science, Google Scholar
- 13. Foo YZ, Lagisz M, O’Dea RE, Nakagawa S. 2023 The influence of immune challenges on the mean and variance in reproductive investment: a meta-analysis of the terminal investment hypothesis. BMC Biol. 21, 107. (doi:10.1186/s12915-023-01603-4) Crossref, PubMed, Web of Science, Google Scholar
- 14. Dvorakova-Hortova K, Sidlova A, Ded L, Hladovcova D, Vieweg M, Weidner W, Steger K, Stopka P, Paradowska-Dogan A. 2014Toxoplasma gondii decreases the reproductive fitness in mice. PLoS ONE 9, e96770. (doi:10.1371/journal.pone.0096770) Crossref, PubMed, Web of Science, Google Scholar
- 15. Garcia‐Ispierto I, Tutusaus J, López‐Gatius F. 2014 Does Coxiella burnetii affect reproduction in cattle? A clinical update. Reprod. Domest. Anim. 49, 529–535. (doi:10.1111/rda.12333) Crossref, PubMed, Google Scholar
- 16. Assis VP, Ribeiro VM, Rachid MA, Castro ACS, Valle GR. 2010 Dogs with Leishmania chagasi infection have semen abnormalities that partially revert during 150 days of allopurinol and amphotericin B therapy. Anim. Reprod. Sci. 117, 183–186. (doi:10.1016/j.anireprosci.2009.03.003) Crossref, PubMed, Web of Science, Google Scholar
- 17. Dalton ADA, Harcourt‐Webster JN. 1991 The histopathology of the testis and epididymis in AIDS—a post‐mortem study. J. Pathol. 163, 47–52. (doi:10.1002/path.1711630109) Crossref, PubMed, Google Scholar
- 18. Aldana M, Pulgar JM, Orellana N, Patricio Ojeda F, García-Huidobro MR. 2014 Increased parasitism of limpets by a trematode metacercaria in fisheries management areas of central Chile: effects on host growth and reproduction. EcoHealth 11, 215–226. (doi:10.1007/s10393-013-0876-9) Crossref, PubMed, Web of Science, Google Scholar
- 19. Bonneaud C, Mazuc J, Chastel O, Westerdahl H, Sorci G. 2004Terminal investment induced by immune challenge and fitness traits associated with major histocompatibility complex in the house sparrow. Evolution 58, 2823. (doi:10.1554/04-279) Crossref, PubMed, Web of Science, Google Scholar
- 20. Kivleniece I, Krams I, Daukšte J, Krama T, Rantala MJ. 2010 Sexual attractiveness of immune-challenged male mealworm beetles suggests terminal investment in reproduction. Anim. Behav. 80, 1015–1021. (doi:10.1016/j.anbehav.2010.09.004) Crossref, Web of Science, Google Scholar
- 21. Lachish S, Jones M, Mccallum H. 2007 The impact of disease on the survival and population growth rate of the Tasmanian devil. J. Anim. Ecol. 76, 926–936. (doi:10.1111/j.1365-2656.2007.01272.x) Crossref, PubMed, Web of Science, Google Scholar
- 22. Roznik EA, Sapsford SJ, Pike DA, Schwarzkopf L, Alford RA. 2015Condition-dependent reproductive effort in frogs infected by a widespread pathogen. Proc. R. Soc. B 282, 20150694. (doi:10.1098/rspb.2015.0694) Link, Web of Science, Google Scholar
- 23. Zuk M. 1996 Disease, endocrine–immune interactions, and sexual selection. Ecology 77, 1037–1042. (doi:10.2307/2265574) Crossref, Web of Science, Google Scholar
- 24. Braude S, Tang-Martinez Z, Taylor GT. 1999 Stress, testosterone, and the immunoredistribution hypothesis. Behav. Ecol. 10, 345–350. (doi:10.1093/beheco/10.3.345) Crossref, Web of Science, Google Scholar
- 25. Folstad I, Karter AJ. 1992 Parasites, bright males, and the immunocompetence handicap. Am. Nat. 139, 603–622. (doi:10.1086/285346) Crossref, Web of Science, Google Scholar
- 26. Scheele BC, Hunter DA, Skerratt LF, Brannelly LA, Driscoll DA. 2015Low impact of chytridiomycosis on frog recruitment enables persistence in refuges despite high adult mortality. Biol. Conserv. 182, 36–43. (doi:10.1016/j.biocon.2014.11.032) Crossref, Web of Science, Google Scholar
- 27. Brannelly LA, Scheele B, Grogan LF. 2020 Disease and the endangered alpine tree frog: bridging research, conservation, and management. In Strategies for conservation success in herpetology (eds Walls S, O’Donnell K). Society for the Study of Amphibians and Reptiles. Google Scholar
- 28. Brannelly LA, Hunter DA, Lenger D, Scheele BC, Skerratt LF, Berger L. 2015 Dynamics of chytridiomycosis during the breeding season in an Australian alpine amphibian. PLoS ONE 10, e0143629. (doi:10.1371/journal.pone.0143629) Crossref, PubMed, Web of Science, Google Scholar
- 29. Brannelly L, Wetzel D, West M, Richards-Zawacki C. 2020Optimized Batrachochytrium dendrobatidis DNA extraction of swab samples results in imperfect detection particularly when infection intensities are low. Dis. Aquat. Org. 139, 233–243. (doi:10.3354/dao03482) Crossref, PubMed, Web of Science, Google Scholar
- 30. Boyle D, Boyle D, Olsen V, Morgan J, Hyatt A. 2004 Rapid quantitative detection of chytridiomycosis (Batrachochytrium dendrobatidis) in amphibian samples using real-time Taqman PCR assay. Dis. Aquat. Org.60, 141–148. (doi:10.3354/dao060141) Crossref, PubMed, Web of Science, Google Scholar
- 31. Jadwani-Bungar T, Doidge NP, Wallace DK, Brannelly LA. 2024Baseline haematological parameters in three common Australian frog species. PeerJ 12, e17406. (doi:10.7717/peerj.17406) Crossref, PubMed, Web of Science, Google Scholar
- 32. Pham TH, Brannelly LA. 2022Sperm parameters following hormonal induction of spermiation in an endangered frog [the alpine tree frog] (Litoria verreauxii alpina). Reprod. Fertil. Dev. 34, 867–874. (doi:10.1071/rd22009) Crossref, PubMed, Web of Science, Google Scholar
- 33. Agarwal A, Gupta S, Sharma R. 2016 Eosin-nigrosin staining procedure. In Andrological evaluation of male infertility (eds Agarwal A, Gupta S, Sharma R), pp. 73–77. Cham, Switzerland: Springer. (doi:10.1007/978-3-319-26797-5_8) Crossref, Google Scholar
- 34. Wang J. 2004 Sibship reconstruction from genetic data with typing errors. Genetics 166, 1963–1979. (doi:10.1093/genetics/166.4.1963) Crossref, PubMed, Web of Science, Google Scholar
- 35. Wang J. 2012 Computationally efficient sibship and parentage assignment from multilocus marker data. Genetics 191, 183–194. (doi:10.1534/genetics.111.138149) Crossref, PubMed, Web of Science, Google Scholar
- 36. Wang J. 2013 A simulation module in the computer program colony for sibship and parentage analysis. Mol. Ecol. Resour. 13, 734–739. (doi:10.1111/1755-0998.12106) Crossref, PubMed, Web of Science, Google Scholar
- 37. Wang J. 2013 An improvement on the maximum likelihood reconstruction of pedigrees from marker data. Heredity 111, 165–174. (doi:10.1038/hdy.2013.34) Crossref, PubMed, Web of Science, Google Scholar
- 38. Jones OR, Wang J. 2010 COLONY: a program for parentage and sibship inference from multilocus genotype data. Mol. Ecol. Resour.10, 551–555. (doi:10.1111/j.1755-0998.2009.02787.x) Crossref, PubMed, Web of Science, Google Scholar
- 39. Jones OR, Wang J. 2010 Molecular marker‐based pedigrees for animal conservation biologists. Anim. Conserv. 13, 26–34. (doi:10.1111/j.1469-1795.2009.00324.x) Crossref, Web of Science, Google Scholar
- 40. RStudio Team. 2020 RStudio: integrated development for R. Boston, MA: RStudio, pbc. See http://www.rstudio.com. Google Scholar
- 41. R Core Team. 2022 R: a language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing. See https://www.R-project.org/. Google Scholar
- 42. Orton F, Roberts‐Rhodes B, Moore E, Whatley C, Tyler CR. 2023Nuptial pad (‘breeding gland’) morphology is related to non‐random mating in wild male common frogs (Rana temporaria). Ethology 129, 232–239. (doi:10.1111/eth.13361) Crossref, Web of Science, Google Scholar
- 43. Gomez D, Richardson C, Lengagne T, Plenet S, Joly P, Léna JP, Théry M. 2009 The role of nocturnal vision in mate choice: females prefer conspicuous males in the European tree frog (Hyla arborea). Proc. R. Soc. B 276, 2351–2358. (doi:10.1098/rspb.2009.0168) Link, Web of Science, Google Scholar
- 44. Trivers RL. 1972 Parental investment and sexual selection. In Sexual selection and the descent of man, pp. 136–179. New York, NY: Aldine de Gruyter. (doi:10.1002/ajpa.1330400226) Google Scholar
- 45. Rosenthal GG, Rand AS, Ryan MJ. 2004 The vocal sac as a visual cue in anuran communication: an experimental analysis using video playback. Anim. Behav. 68, 55–58. (doi:10.1016/j.anbehav.2003.07.013) Crossref, Web of Science, Google Scholar
- 46. Longo AV, Rodríguez‐Gómez CA, Zegarra JP, Monzón O, Claudio‐Hernández HJ, Joglar RL, Zamudio KR, Burrowes PA, López‐Torres AL. 2020 Tick parasitism as a cost of sexual selection and male parental care in a Neotropical frog. Ecosphere 11, e03010. (doi:10.1002/ecs2.3010) Crossref, Web of Science, Google Scholar
- 47. Kellie A, Dain SJ, Banks PB. 2004Ultraviolet properties of Australian mammal urine. J. Comp. Physiol.190, 429–435. (doi:10.1007/s00359-004-0507-6) Crossref, Web of Science, Google Scholar
- 48. Bajer K, Molnár O, Török J, Herczeg G. 2011 Ultraviolet nuptial colour determines fight success in male European green lizards (Lacerta viridis). Biol. Lett. 7, 866–868. (doi:10.1098/rsbl.2011.0520) Link, Web of Science, Google Scholar
- 49. Whiting MJ, Stuart-Fox DM, O’Connor D, Firth D, Bennett NC, Blomberg SP. 2006 Ultraviolet signals ultra-aggression in a lizard. Anim. Behav. 72, 353–363. (doi:10.1016/j.anbehav.2005.10.018) Crossref, Web of Science, Google Scholar
- 50. Ries C, Spaethe J, Sztatecsny M, Strondl C, Hödl W. 2008 Turning blue and ultraviolet: sex‐specific colour change during the mating season in the Balkan moor frog. J. Zool. 276, 229–236. (doi:10.1111/j.1469-7998.2008.00456.x) Crossref, Web of Science, Google Scholar
- 51. Yovanovich CAM, Pierotti MER, Kelber A, Jorgewich-Cohen G, Ibáñez R, Grant T. 2020 Lens transmittance shapes ultraviolet sensitivity in the eyes of frogs from diverse ecological and phylogenetic backgrounds. Proc. R. Soc. B 287, 20192253. (doi:10.1098/rspb.2019.2253) Link, Web of Science, Google Scholar
- 52. Stegen JC, Gienger CM, Sun L. 2004 The control of color change in the Pacific tree frog, Hyla regilla. Can. J. Zool. 82, 889–896. (doi:10.1139/z04-068) Crossref, Web of Science, Google Scholar
- 53. Hettyey A, Herczeg G, Laurila A, Crochet PA, Merilä J. 2009 Body temperature, size, nuptial colouration and mating success in male moor frogs (Rana arvalis). Amphib. Reptil. 30, 37–43. (doi:10.1163/156853809787392784) Crossref, Web of Science, Google Scholar
- 54. Sztatecsny M, Preininger D, Freudmann A, Loretto MC, Maier F, Hödl W. 2012 Don’t get the blues: conspicuous nuptial colouration of male moor frogs (Rana arvalis) supports visual mate recognition during scramble competition in large breeding aggregations. Behav. Ecol. Sociobiol. 66, 1587–1593. (doi:10.1007/s00265-012-1412-6) Crossref, PubMed, Web of Science, Google Scholar
- 55. Pérez i de Lanuza G, Carazo P, Font E. 2014 Colours of quality: structural (but not pigment) coloration informs about male quality in a polychromatic lizard. Anim. Behav. 90, 73–81. (doi:10.1016/j.anbehav.2014.01.017) Crossref, Web of Science, Google Scholar
- 56. McGraw KJ, Mackillop EA, Dale J, Hauber ME. 2002 Different colors reveal different information: how nutritional stress affects the expression of melanin- and structurally based ornamental plumage. J. Exp. Biol. 205, 3747–3755. (doi:10.1242/jeb.205.23.3747) Crossref, PubMed, Web of Science, Google Scholar
- 57. Bajer K, Molnár O, Török J, Herczeg G. 2012 Temperature, but not available energy, affects the expression of a sexually selected ultraviolet (UV) colour trait in male European green lizards. PLoS ONE7, e34359. (doi:10.1371/journal.pone.0034359) Crossref, PubMed, Web of Science, Google Scholar
- 58. Liao WB, Huang Y, Zeng Y, Zhong MJ, Luo Y, Lüpold S. 2018 Ejaculate evolution in external fertilizers: influenced by sperm competition or sperm limitation? Evolution 72, 4–17. (doi:10.1111/evo.13372) Crossref, PubMed, Web of Science, Google Scholar
- 59. Della Togna G, Trudeau VL, Gratwicke B, Evans M, Augustine L, Chia H, Bronikowski EJ, Murphy JB, Comizzoli P. 2017 Effects of hormonal stimulation on the concentration and quality of excreted spermatozoa in the critically endangered Panamanian golden frog (Atelopus zeteki). Theriogenology 91, 27–35. (doi:10.1016/j.theriogenology.2016.12.033) Crossref, PubMed, Web of Science, Google Scholar
- 60. Voyles J et al.. 2009 Pathogenesis of chytridiomycosis, a cause of catastrophic amphibian declines. Science 326, 582–585. (doi:10.1126/science.1176765) Crossref, PubMed, Web of Science, Google Scholar
- 61. García-Gonzá Lez F, Simmons L. 2005 Sperm viability matters in insect sperm competition. Curr. Biol.15, 271–275. (doi:10.1016/j)) Crossref, PubMed, Google Scholar
- 62. Sherman CDH, Uller T, Wapstra E, Olsson M. 2008 Within-population variation in ejaculate characteristics in a prolonged breeder, Peron’s tree frog, Litoria peronii. Naturwissenschaften 95, 1055–1061. (doi:10.1007/s00114-008-0423-7) Crossref, PubMed, Google Scholar
- 63. Byrne PG, Simmons LW, Roberts JD. 2003 Sperm competition and the evolution of gamete morphology in frogs. Proc. R. Soc. Lond. B 270, 2079–2086. (doi:10.1098/rspb.2003.2433) Link, Web of Science, Google Scholar
- 64. Rastogi RK, Iela L, Meglio M, Fiore M, D’Aniello B, Pinelli C, Fiorentino M. 2005 Hormonal regulation of reproductive cycles in amphibians. In Amphibian biology, pp. 2045–2177. Chipping Norton, Australia: Surrey Beatty & Son. Google Scholar
- 65. Teacher AGF, Garner TWJ, Nichols RA. 2009 Population genetic patterns suggest a behavioural change in wild common frogs (Rana temporaria) following disease outbreaks (Ranavirus). Mol. Ecol. 18, 3163–3172. (doi:10.1111/j.1365-294X.2009.04263.x) Crossref, PubMed, Web of Science, Google Scholar
- 66. Scheele BC, Foster CN, Hunter DA, Lindenmayer DB, Schmidt BR, Heard GW. 2019 Living with the enemy: facilitating amphibian coexistence with disease. Biol. Conserv. 236, 52–59. (doi:10.1016/j.biocon.2019.05.032) Crossref, Web of Science, Google Scholar
- 67. Brannelly LA. 2024 Devastating disease can cause increased breeding effort and success that improves population resilience. Dryad Digital Repository. (doi:10.5061/dryad.vx0k6dk0f) Google Scholar
- 68. Brannelly LA, Wallace D, Wendt A, Higgs Q, Zhang S, Hough M et al.. 2025 Supplementary material from: Devastating disease can cause increased breeding effort and success that improves population resilience. Figshare. (doi:10.6084/m9.figshare.c.7821008) Google Scholar